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Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
An activator of G6PDH (EC50 = 3.4 µM for G6PDHR459L); selectively induces the dimerization of G6PDHR459L over 6PGD, GAPDH, ALDH2, and ALDH3A1; decreases ROS levels in, and increases the viability of, primary human lymphocytes isolated from a patient carrying the G6PDHR459L mutation; inhibits chloroquine- or diamide-induced hemolysis in isolated human erythrocytes at 5 µM; decreases chloroquine-induced pericardial edema in zebrafish embryos at 1 µM; decreases tumor volume and weight and increases survival in an NCI H226 lung mesothelioma mouse xenograft model at 40 mg/kg
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GoldBio s GV3101 electrocompetent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species such as Arabidopsis thaliana tobacco potato and corn These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome Additionally the cells are resistant to rifampicin gentamicin and tetracycline which allows for selection of transformed cells
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Intact Genomics SS320 (MC1061F\’) phage display electrocompetent cells are suitable for protein expression, general cloning, blue/white screening, M13 phage work and phage display protein expression.Intact Genomics SS320 phage display electrocompetent cells have the following features: • >5 x 10^10cfu/µg efficiency with electroporation. • Non-amber suppressor strain (sometimes called MC1061F\’)Product Include and Storage: SS320 phage display electrocompetent cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4ºC
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GoldBio s BL21 (DE3) Electrocompetent E coli cells are suitable for high efficiency transformation and routine protein expression Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E coli cells ideal for construction of complex expression libraries and feature 1 x 10 8 cfu/ g efficiency with electroporation BL21 (DE3) Electrocompetent E coli cells have a widely used background feature a T7 expression strain are deficient in both lon (1) and ompT proteases These cells are resistant to phage T1( fhuA2 ) and are B strain
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GoldBio’s GB10B-Pro™ Electrocompetent E. coli cells are especially designed for the most demanding cloning applications. GB10B-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.
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GoldBio s GB10B Chemically Competent E coli cells are equivalent to DH10B cells GB10B cells are high efficiency and suitable for a wide variety of applications such as cloning and sub-cloning GB10B Chemically Competent E coli cells have multiple features including the 80lacZ M15 marker which provides -complementation of the -galactosidase gene with blue/white screening protocol These cells also have the mcrA genotypic marker and the mcrBC mrr deletion which allows for cloning of DNA that contains methylcytosine and methyladenine
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
Chemically competent E. coli cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.
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Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.Lucigens OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
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GoldBio s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region The binary system makes possible to transfer genetic material into a host plant s genome Our system is often used for Agrobacterium -mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana tobacco potato soybeans and corn
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